Detection of EGFR and KRAS mutations in fine-needle aspirates stored on Whatman FTA cards
نویسندگان
چکیده
منابع مشابه
EGFR and K-ras mutations in cytologic samples from fine-needle aspirates in NSCLC patients.
1 Bush A, Hedlin G, Carlsen KH, et al. Severe childhood asthma: a common international approach? Lancet 2008; 372: 1019–1021. 2 Hedlin G, Bush A, Lødrup Carlsen K, et al. Problematic severe asthma in children, not one problem but many: a GALEN initiative. Eur Respir J 2010; 36: 196–201. 3 Lang AM, Konradsen J, Carlsen KH, et al. Identifying problematic severe asthma in the individual child – do...
متن کاملDNA elution from buccal cells stored on Whatman FTA Classic Cards using a modified methanol fixation method.
We describe here a method for DNA elution from buccal cells and whole blood both collected onto Whatman FTA technology, using methanol fixation followed by an elution PCR program. Extracted DNA is comparable in quality to published Whatman FTA protocols, as judged by PCR-based genotyping. Elution of DNA from the dried sample is a known rate-limiting step in the published Whatman FTA protocol; t...
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OBJECTIVES To determine the utility of the cell transfer technique (CTT) for BRAF molecular testing on thyroid fine-needle aspiration (FNA) specimens. METHODS Polymerase chain reaction (PCR)-based BRAF molecular testing was performed on tissues obtained through CTT from both air-dried and ethanol-fixed direct smears of thyroid FNA specimens and then compared with the corresponding thyroidecto...
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To determine the frequency and distribution of mitochondrial DNA mutations in breast cancer, 18 primary breast tumors were analyzed by direct sequencing. Twelve somatic mutations not present in matched lymphocytes and normal breast tissues were detected in 11 of the tumors screened (61%). Of these mutations, five (42%) were deletions or insertions in a homopolymeric C-stretch between nucleotide...
متن کاملDetection of c-K-ras mutations in fine needle aspirates from human pancreatic adenocarcinomas.
Formalin-fixed paraffin-embedded tissue specimens obtained by fine needle aspiration of pancreatic masses from 47 patients were examined retrospectively for cytology and the presence of mutant c-K-ras oncogenes. Point mutations of c-K-ras in codon 12 were detected by RNA-DNA RNAse A mismatch cleavage after in vitro DNA amplification of the cellular c-K-ras sequences by the polymerase chain reac...
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ژورنال
عنوان ژورنال: Cancer Cytopathology
سال: 2010
ISSN: 1934-662X
DOI: 10.1002/cncy.20102